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Liquid Chromatography: Thin Layer Chromatography Background reading – Mohrig Chapter 18 (Thin-layer chromatography) Safety The TLC developing solution is (25:1:1 ethyl acetate, ethanol, acetic acid) Ethanol and ethyl acetate are toxic compounds. Acetic acid can be irritating to the skin, and has an unpleasant odor. To avoid contact with the skin, wear gloves as much as possible. If any of the compounds or solutions is spilled on the skin, wash the affected area thoroughly with cold water and soap. The analgesics are: acetaminophen, aspirin, caffeine, and ibuprofen. Work in the hood to avoid breathing the vapors of ethanol, ethyl acetate, and acetic acid. Procedure Preparation of TLC materials 1) Prepare six to eight TLC capillaries from melting point capillaries. DO NOT WEAR GLOVES WHILE PULLING CAPILLARIES! 2) Cut two pieces of filter paper

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